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Production of Antihypertensive Elastin Peptides from Waste Poultry Skin

Salma Mohamad Yusop 1, Mehdi Nadalian 1, Mehdi Nadalian 1, Wan Aida Wan Mustapha 1, Bita Forghani 1, and Mohd Azri Azman 2
1. School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM, Bangi, Selangor, Malaysia
2. Strategic Livestock Research Centre, Malaysian Agricultural Research and Development Institute, MARDI Headquarters, 43400, Serdang, Selangor, Malaysia
Abstract—Poultry by-products are not often processed into high-value products. Chicken skin can be considered as one of the most under-utilized by-products and usually been removed for its stigma of high fat content. However, considering that it contains 10-22% protein, poultry skin could be utilized to produce elastin, which is often being incorporated in the production of functional food, cosmetics industry and regenerative medicine. Extracting elastin provides a huge challenge but even bigger opportunities as it has been proven beneficial in the production of functional food. In this study, water-soluble elastin was successfully extracted from broiler and spent hen skin using four different solvents including NaCl, acetone, NaOH and oxalic acid (solubilizing liquid) prior to freeze-drying. The extracted elastin was tested to confirm the purity using amino acid profiling. The water-soluble elastin was analyzed for ACE inhibitory and SDS-PAGE test. It was evident that extracted methods produced high quality elastin comparable to that of commercial elastin, with low amount of Methionine and Histidine. ACE inhibitory activity is present in chicken skin hydrolysate. The ultra-filtrated elastin fraction of broiler and spent hen skin hydrolysate with size ≤3KDa has highest ACE inhibitory activity. The results of the study are the first report on the elastin extracted from broiler and spent hen skin hydrolysis with commercial enzymes could provide the peptides with ACE inhibitory activities. In conclusion, elastin from poultry skin could be a potential new source of natural antioxidant and antihypertensive ingredient for use in food, drinks and cosmetics application. 
 
Index Terms—elastin, extraction, broiler skin, spent hen skin, ACE inhibitory activity

Cite: Salma Mohamad Yusop, Mehdi Nadalian, Abdul Salam Babji, Wan Aida Wan Mustapha, Bita Forghani, and Mohd Azri Azman, "Production of Antihypertensive Elastin Peptides from Waste Poultry Skin," International Journal of Food Engineering, Vol. 2, No. 1, pp. 21-25, June 2016. doi: 10.18178/ijfe.2.1.21-25
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