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Kinetics and Nondestructive Measurement of Total Volatile Basic Nitrogen and Thiobarbituric Acid-Reactive Substances in Chilled Tabtim Fish Fillets Using Near Infrared Spectroscopy (NIRS)

Sutee Wangtueai 1, Chaluntorn Vichasilp 2, Tanachai Pankasemsuk 3, Parichart Theanjumpol 3, and Yuthana Phimolsiripol 1
1. Faculty of Agro-Industry, Chiang Mai University, Mae-Hea, Muang, Chiang Mai 50100, Thailand
2. Faculty of Natural Resources, Rajamangala University of Technology Isan, Sakon Nakhon 47160, Thailand
3. Postharvest Technology Research Institute, Chiang Mai University, Muang, Chiang Mai 50200, Thailand
Abstract—Tabtim fish (Oreochromis sp.) fillets were prepared with a size 100-150g/piece, individual packed into zip lock polyethylene bag and kept at 0, 5, and 10C up to 18 days. Total volatile basic nitrogen (TVB-N) and thiobarbituric acid-reactive substances (TBARS) were determined. The TVB-N and TBARS changes during storage were found to be adequately described by first-order reaction kinetics. The NIR spectra of TVB-N and TBARS in fish fillets (180 samples) were collected in the reflectance mode using NIRSystems 6500 at 25C. Results showed that the TVB-N and TBARS were 9.40-99.7mg/100g and 0.13-2.06mg malondialdehyde/kg, respectively. The spectra were in range of short-wavelength region (700-1000nm) and long-wavelength region (1000-2500nm). Consequently, the partial regression model with cross validation was created and the optimized model was done with full NIR spectra, short wavelength and long wavelength. It was found that the acceptable model of TVB-N was obtained with 750-1000nm with determination coefficient of calibration (R2cal) = 0.78. A model of TBARS showed good data with short wavelength (750-1000nm). Considering the obtained results, it is suggested that the TVB-N model could be used as a screening step to determine the TVB-N of Tabtim fish fillets.
 
Index Terms—near infrared spectroscopy, Tabtim fish (Oreochromis sp.), fish fillets, TVB-N, TBARS

Cite: Sutee Wangtueai, Chaluntorn Vichasilp, Tanachai Pankasemsuk, Parichart Theanjumpol, and Yuthana Phimolsiripol, "Kinetics and Nondestructive Measurement of Total Volatile Basic Nitrogen and Thiobarbituric Acid-Reactive Substances in Chilled Tabtim Fish Fillets Using Near Infrared Spectroscopy (NIRS)," International Journal of Food Engineering, Vol. 2, No. 1, pp. 16-20, June 2016. doi: 10.18178/ijfe.2.1.16-20
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