Abstract—Yeast exopolysaccharide (EPS) are composed of unique sugar units, which offers an interesting perspective with respect to various food applications. This study aimed to characterize the EPS from Rhodotorula minuta BIOTECH 2178. Toxicological assessment of the EPS was investigated to ensure that it is safe for human consumption. The yeast EPS revealed high total sugar composition (59.21-62-47%) and low protein content (17.19%). FTIR spectrum showed prominent functional group such as hydroxyl, carboxyl, beta-linkages, glucose, mannan, and aromatic ring. SEM micrograph of EPS revealed a highly compact with elongated granular structures. Toxicological examination of EPS (50-400mg EPS/kg bodyweight) did not show any symptoms of toxicity and mortality after 14 days administration of aqueous crude EPS. Hematological and histopathological examination in the liver showed no significant alteration between the experimental and control groups. Hence, the highly compact and unique functional properties of Rhodotorula minuta EPS showed its potential application in the industry as thickening and stabilizing agent.