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Study & Application on the Enzyme-Linked Immunosorbent Assay System of Grain in Southern China

Wenhui Li 1, Jiadong Chen 1, Li Liu 2, Huiyong Li 3, and Ting Xu 4
1. Guangdong Institute for Cereal Science Research, Guangzhou, China
2. Guangzhou Automobile Group Co. Ltd., Guangzhou, China
3. Cornell University, Ithaca, NY 14850, United States
4. College of Resources and Environmental Sciences, China Agricultural University, Beijing, China
Abstract—With the acceleration of economic globalization, food security is not only related to the health of consumers, but also affects the international food and agricultural trade. It has become one of significant global focus. Aiming at the safety issues of chemical contamination in food processing, storage and transportation, the rapid detection technology of food security is studied in this paper, while four kinds of hapten with four kinds of antibodies were designed and screened. The effective detection reagents including PBDEs, imidacloprid, polybrominated diphenyl ether, bifenthrin, and fenvalerate were evaluated. The results show that the lowest detection limit (IC10) for the detection of imidacloprid, polybrominated diphenyl ethers, fenvalerate and fenvalerate were 1.8ng/g, 0.1ng/g, 4.7ng/g, and 1.0ng/g. Through the correlation analysis, grain, rice and corn samples were highly correlated based on the average detection time, which was less than 10 minutes, faster than Gas Chromatography (GC) method.
Index Terms—detection reagents, IC10, correlation analysis, GC method1

Cite: Wenhui Li, Jiadong Chen, Li Liu, Huiyong Li, and Ting Xu, "Study & Application on the Enzyme-Linked Immunosorbent Assay System of Grain in Southern China," International Journal of Food Engineering, Vol. 4, No. 2, pp. 101-109, June 2018. doi: 10.18178/ijfe.4.2.101-109
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